OPTIMIZED PROTOCOL FOR ISOLATION OF HIGH-QUALITY SINGLE CELLS FROM THE FEMALE MOUSE REPRODUCTIVE TRACT TISSUES FOR SINGLE-CELL RNA SEQUENCING

Optimized protocol for isolation of high-quality single cells from the female mouse reproductive tract tissues for single-cell RNA sequencing

Summary: Single-cell RNA sequencing (scRNA-seq) is a powerful tool for enumerating the gene expression dynamics at single-cell resolution.Various organs comprising distinct cellular composition and architecture Cocktail Napkins require unique approaches for highly viable single-cell preparation and reliable sequencing results.Here, we describe an o

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Acetic Acid as Processing Aid Dramatically Improves Organic Solvent Solubility of Weakly Basic Drugs for Spray Dried Dispersion Manufacture

Many active pharmaceutical ingredients (APIs) in the pharmaceutical pipeline require bioavailability enhancing formulations due Goalie - Sticks - Junior to very low aqueous solubility.Although spray dried dispersions (SDDs) have demonstrated broad utility in enhancing the bioavailability of such APIs by trapping them in a high-energy amorphous form

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Sensitivity comparison between rapid one-step test strip and ELISA methods in detection of HBsAg among selected chronic liver disease patients in University of Calabar Teaching hospital, Calabar, Nigeria

Background: Hepatitis B virus (HBV) is the eloquent hepatic infection that leads to hepatocellular carcinoma.This study aims to determine the frequency of HBV by screening selected chronic liver disease (CLD) patients alongside few apparently healthy subjects who might be carriers and pose a high risk in HBV transmission.This study also aims to com

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